What type of cells can FISH be performed on?

Fluorescence in situ hybridization (FISH) is a technique that allows for the identification and localization of specific DNA sequences on chromosomes. This method can be performed on both uncultured cells, such as interphase cells taken directly from tissues or blood, and cultured cells, particularly those that are in metaphase.

Using both uncultured and cultured cells makes FISH a versatile tool in cytogenetics, as it can provide insights into chromosomal abnormalities in a variety of contexts. For instance, uncultured cells are often used in prenatal diagnosis, while cultured metaphase cells may be used for detailed chromosomal analyses. Thus, the ability to utilize FISH on both types of cells enhances its clinical utility across different scenarios, including cancer diagnosis and prenatal testing.